-correction of magnetization transfer saturation maps optimized for 7T postmortem MRI of the brain

Magnetization transfer saturation ( $$ ) is a useful marker to probe tissue macromolecular content and myelination in the brain. The increased $$ -inhomogeneity at $$  T and significantly larger saturation pulse flip angles which are often used for postmortem studies exceed the limits where previous $$ $$ correction methods are applicable. Here, we develop a calibration-based correction model and procedure, and validate and evaluate it in postmortem 7T data of whole chimpanzee brains.

The $$ dependence of $$ was investigated by varying the off-resonance saturation pulse flip angle. For the range of saturation pulse flip angles applied in typical experiments on postmortem tissue, the dependence was close to linear. A linear model with a single calibration constant $$ is proposed to correct bias in $$ by mapping it to the reference value of the saturation pulse flip angle.

$$ was estimated voxel-wise in five postmortem chimpanzee brains. "Individual-based global parameters" were obtained by calculating the mean $$ within individual specimen brains and "group-based global parameters" by calculating the means of the individual-based global parameters across the five brains.

The linear calibration model described the data well, though $$ was not entirely independent of the underlying tissue and $$ . Individual-based correction parameters and a group-based global correction parameter ( $$ ) led to visible, quantifiable reductions of $$ -biases in high-resolution $$ maps.

The presented model and calibration approach effectively corrects for $$ inhomogeneities in postmortem 7T data.