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Amino Acids Profile in Children with Acute Brucellosis
Ahmet Guzelcicek1, Nihayet Bayraktar2, Mehmet Bayraktar3
1Department of Pediatrics, Harran University, Medical School, Sanliurfa, Turkey.
Current Pediatric Reviews
|November 16, 2022
Summary
Brucellosis infection significantly alters plasma amino acid profiles, revealing specific metabolic changes. This study identifies key amino acid differences in patients with brucellosis.
Area of Science:
- Biochemistry
- Infectious Diseases
- Metabolomics
Background:
- Brucellosis is a significant public health concern in Turkey, often peaking in spring.
- Consumption of unpasteurized dairy products is a primary transmission route.
- Amino acid profiles in brucellosis have not been previously investigated.
Purpose of the Study:
- To investigate the plasma amino acid profile in patients with acute brucellosis.
- To determine if infectious diseases like brucellosis impact amino acid levels.
- To establish a baseline amino acid profile for brucellosis patients.
Main Methods:
- Plasma samples were analyzed using an 8045 LC-MS/MS device.
- The JASEM amino acid kit was employed for sample preparation and analysis.
- Quantitative analysis of 45 amino acids was performed.
Main Results:
- Significant differences in the concentrations of 25 amino acids were observed in brucellosis patients compared to controls.
- Specific amino acids like alanine, arginine, and taurine showed significant alterations (p < 0.05).
- No significant changes were found for 20 other amino acids (p > 0.05).
Conclusions:
- Brucellosis patients exhibit a distinct plasma amino acid profile.
- These alterations suggest underlying pathological and metabolic changes caused by Brucella spp. infection.
- The findings highlight the potential of amino acid profiling in understanding brucellosis's biochemical impact.
