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Fluorescence Lifetime Macro Imager for Biomedical Applications

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Ion Microscope Imaging Mass Spectrometry Using a Timepix3-Based Optical Camera.

Daniel Wood¹, Robert J Burleigh², Natasha Smith²

¹Robert Hooke Building, Department of Physics, University of Oxford, Parks Road, OxfordOX1 3PP, United Kingdom.

Journal of the American Society for Mass Spectrometry

|November 16, 2022

Summary

This summary is machine-generated.

This study introduces a novel ion microscope mass spectrometer with precise timing for high-throughput imaging. The system utilizes a Timepix3 readout and a unique triggering method for improved mass resolution and cost-effectiveness.

Keywords:

fast imaging sensor ion microscope imaging mass spectrometry imaging stigmatic ion imaging

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Area of Science:

Analytical Chemistry
Microscopy
Mass Spectrometry

Background:

High-throughput mass spectrometry imaging requires precise detector timing to resolve complex mass spectra.
Existing systems often need external, costly time-to-digital converters (TDCs).

Purpose of the Study:

To present a new ion microscope mass spectrometer with enhanced timing resolution.
To demonstrate a cost-effective readout system for mass spectrometry imaging.

Main Methods:

Utilized a Timepix3 hybrid pixel readout for optimal 1.56 ns timing resolution.
Implemented a novel triggering technique to eliminate the need for an external TDC.
Applied multimass imaging sensors for high-resolution mass spectrometry imaging.

Main Results:

Achieved high mass resolution in spectrometry imaging.
Demonstrated the system's effectiveness using rhodamine B samples.
Showcased a low-cost, commercially available readout system.

Conclusions:

The developed ion microscope enables high-throughput, high-mass-resolution imaging.
The novel triggering technique simplifies the experimental setup and reduces costs.
Multimass imaging sensors are effective for advanced microscopy mass spectrometry.