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Updated: Aug 21, 2025

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Published on: December 31, 2017

Skin microbiome sampling in the preterm neonate.

Jennifer J Schoch¹, Josee Gauthier², Raad Z Gharaibeh³

¹Department of Dermatology, University of Florida College of Medicine, Gainesville, Florida, USA.

Pediatric Dermatology

|November 17, 2022

Summary

This summary is machine-generated.

This study analyzed the skin microbiome in preterm infants, finding that samples from the same source (skin, crib, or control) were more alike than samples taken over time or from different infants. This highlights the importance of sampling location in skin microbiome research.

Keywords:

cutaneous microbiome neonatal intensive care unit prematurity preterm skin microbiome

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Area of Science:

Microbiology
Neonatal Health
Dermatology

Background:

The human microbiome is increasingly understood, yet the skin microbiome in preterm neonates remains understudied.
Preterm infants may have unique skin microbiome compositions due to their environment and developmental stage.

Purpose of the Study:

To characterize the skin microbiome of preterm neonates.
To compare preterm infant skin microbiome samples with environmental and control samples.
To investigate the influence of sampling time and patient on microbiome similarity.

Main Methods:

Collected skin microbiome samples from six preterm neonates across multiple time points.
Collected environmental samples (crib/isolette swabs) and negative controls.
Utilized comparative analysis to assess microbiome similarity based on sample type, time, and patient.

Main Results:

Microbiome samples of the same type (skin, crib, or control) exhibited higher similarity to each other.
Similarity was greater within sample types compared to similarity across different weeks or between different patients.
This suggests the sampling location is a dominant factor in observed microbial community structure.

Conclusions:

The study provides initial insights into the preterm neonate skin microbiome.
Environmental factors and sampling consistency are crucial considerations in studying the preterm infant skin microbiome.
Further research is needed to fully elucidate the dynamics and implications of the preterm skin microbiome.