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Updated: Aug 21, 2025

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

Published on: February 2, 2011

Autologous NK cells propagated and activated ex vivo decrease senescence markers in human PBMCs.

Nickolas Chelyapov¹, Toai T Nguyen¹, Rafael Gonzalez²

¹Thebiobox, LLC, 355 East Rincon Street, Suite 125, Corona, CA, 92879, USA.

Biochemistry and Biophysics Reports

|November 17, 2022

Summary

This summary is machine-generated.

Autologous activated natural killer (aNK) cells reduce senescent cells and immunosenescence in vitro and in humans. This finding suggests aNK cells can mitigate aging-related inflammation by clearing senescent cells.

Keywords:

Aging Inflammation NK cells Senescence aNK, Autologous propagated and activated natural killer cells

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Published on: April 23, 2013

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Last Updated: Aug 21, 2025

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

Published on: February 2, 2011

Generation of Natural Killer Cells from Human Expanded Potential Stem Cells

Generation of Natural Killer Cells from Human Expanded Potential Stem Cells

Published on: January 13, 2023

Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells hESCs and Induced Pluripotent Stem Cells iPSCs

Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells hESCs and Induced Pluripotent Stem Cells iPSCs

Published on: April 23, 2013

Area of Science:

Immunology
Gerontology
Cell Biology

Background:

Aging involves senescence and chronic inflammation, negatively impacting the immune system.
Natural killer (NK) cells are key innate immune cells involved in eliminating senescent cells.

Purpose of the Study:

To investigate the efficacy of autologous activated NK (aNK) cells in reducing senescent cells and immunosenescence.
To explore the potential of aNK cells in mitigating aging-related inflammation.

Main Methods:

Utilized autologous propagated and activated NK (aNK) cells from five patients.
Assessed the reduction of senescent cells in vitro using markers p16 and β-galactosidase.
Analyzed inflammatory cytokine panels to evaluate the impact on aging-related inflammation.

Main Results:

Demonstrated that aNK cells significantly decrease senescent cells in vitro.
Showed a reduction in immunosenescence markers in human samples treated with aNK cells.
Inflammatory cytokine data indicated a potential role for aNK cell-mediated clearance in reducing aging-related inflammation.

Conclusions:

Autologous activated NK cells effectively reduce senescent cells and markers of immunosenescence.
Targeting senescent cells with aNK cells may offer a therapeutic strategy to combat age-related inflammation.