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Updated: Aug 20, 2025

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Capacitive immunosensor for COVID-19 diagnosis.

Isabella Sampaio¹, Nayla Naomi Kusimoto Takeuti¹, Beatriz Gusson¹

¹GNano - Nanomedicine and Nanotoxicology Group, São Carlos Institute of Physics, University of São Paulo CP 369, 13560-970 São Carlos, SP, Brazil.

Microelectronic Engineering

|November 21, 2022

Summary

This summary is machine-generated.

A new capacitive immunosensor detects SARS-CoV-2 spike proteins for COVID-19 diagnosis. This device offers a reliable alternative, distinguishing it from Dengue and Zika virus proteins, crucial for co-endemic regions.

Keywords:

COVID-19 diagnosis Capacitive biosensor Immunosensor Spike protein detection

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Last Updated: Aug 20, 2025

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

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A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

Published on: July 5, 2022

Area of Science:

Biomedical Engineering
Infectious Disease Diagnostics
Biosensor Technology

Background:

Global spread of COVID-19 necessitates rapid and reliable diagnostic tools.
High demand for diagnostic tests can lead to supply shortages.
Co-circulation of Dengue and Zika viruses presents diagnostic challenges due to similar symptoms and cross-reactivity.

Purpose of the Study:

To develop a qualitative immunosensor for the detection of SARS-CoV-2 spike proteins.
To create a diagnostic tool that can differentiate between SARS-CoV-2, Dengue, and Zika virus proteins.
To enable point-of-care detection for COVID-19, especially in tropical regions.

Main Methods:

Development of a capacitive immunosensor utilizing interdigitated electrodes.
Capacitive detection of spike proteins from SARS-CoV-2, and NS1 proteins from Dengue and Zika viruses.
Analysis of capacitance variation at a 1 kHz frequency to differentiate target proteins.

Main Results:

The immunosensor demonstrated a good signal-to-noise ratio at 1 kHz.
Significantly different capacitance variations were observed: |ΔC| = 7.0 ± 1.8 nF for SARS-CoV-2 spike protein versus |ΔC| = 1.5 ± 1.0 nF for Dengue NS1 and 1.8 ± 1.0 nF for Zika NS1.
A cut-off value of |ΔC| > 3.8 nF was established to indicate the presence of target proteins.

Conclusions:

The developed immunosensor effectively detects SARS-CoV-2 spike proteins.
The sensor shows high specificity, distinguishing SARS-CoV-2 from Dengue and Zika virus proteins.
The miniaturized design and simple electrical circuit enable potential point-of-care applications for COVID-19 diagnosis.