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A sensitive sensing system based on fluorescence dipeptide nanoparticles for sulfadimethoxine determination
Rongfang Yan1, Zhenhua Wen1, Xuelian Hu1
1College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China.
Food Chemistry
|November 22, 2022
Summary
A new biodegradable fluorescent nanoparticle sensor was developed for detecting sulfadimethoxine in food. This method offers a sensitive and reliable way to analyze veterinary drug residues in liquid food samples.
Area of Science:
- Analytical Chemistry
- Nanotechnology
- Biochemistry
Background:
- Fluorescent nanoparticles are used for detecting veterinary drug residues in food.
- Existing methods lack biodegradable nanoparticles that can conjugate with molecular recognition elements.
Purpose of the Study:
- To develop a biodegradable fluorescent nanoparticle-based sensing strategy for sulfadimethoxine detection.
- To create a reliable method for analyzing sulfadimethoxine in liquid food matrices.
Main Methods:
- Assembled a biodegradable tryptophan-phenylalanine dipeptide-based nanomaterial (TPNPs).
- Modified TPNPs with a sulfadimethoxine aptamer (TPNPs@aptamer).
- Developed a fluorescence sensing strategy using TPNPs@aptamer and BHQ1-labeled single-stranded DNA (BHQ1@cDNA).
Main Results:
- Achieved linear fluorescence detection of sulfadimethoxine in the range of 0-200 ng/mL.
- Established a detection limit of 1.47 ng/mL (S/N = 3).
- Obtained satisfactory recoveries (92.88%–114.15%) with relative standard deviation < 5.0% in water and milk samples.
Conclusions:
- The proposed fluorescence sensing strategy is effective for sulfadimethoxine determination.
- The biodegradable TPNPs@aptamer system shows promise for reliable analysis of veterinary drug residues in liquid foods.
