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Using Blue Mini-LEDs as a Light Source Designed a Miniaturized Optomechanical Device for the Detection of Direct
Zhi Ting Ye1, Hsin-Ching Kuo2, Shen Fu Tseng3
1Department of Mechanical Engineering, Advanced Institute of Manufacturing with High-Tech Innovations, National Chung Cheng University, 168, University Rd., Min-Hsiung, Chia-Yi, 62102, Taiwan, ROC. imezty@ccu.edu.tw.
Nanoscale Research Letters
|November 23, 2022
Summary
This study presents a miniaturized optomechanical device (MOD) for noninvasively detecting direct bilirubin in urine. Using blue mini-light-emitting diodes (Mini-LEDs) and optical spectroscopy, the device offers a feasible alternative to invasive blood tests.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Spectroscopy
Background:
- Direct bilirubin is a key biomarker for liver function.
- Current detection methods often involve invasive blood sampling.
- A noninvasive, spectroscopic method for direct bilirubin detection is needed.
Purpose of the Study:
- To develop and validate a miniaturized optomechanical device (MOD) for direct bilirubin detection in urine.
- To utilize blue mini-light-emitting diodes (Mini-LEDs) as a light source for optical spectroscopy.
- To establish a noninvasive method for quantifying direct bilirubin concentrations.
Main Methods:
- Construction of a miniaturized optomechanical device (MOD).
- Application of optical spectroscopy using blue Mini-LEDs (462 nm) to analyze urine samples.
- Measurement of absorbance spectra across varying direct bilirubin concentrations.
Main Results:
- A strong correlation (R² = 0.9999) was observed between absorbance and direct bilirubin concentration (0.855–17.1 μmol/L).
- The device achieved a limit of detection (LOD) of 0.171 μmol/L.
- The limit of quantitation (LOQ) was determined to be 0.570 μmol/L.
Conclusions:
- The developed MOD with blue Mini-LEDs is effective for spectroscopic detection of direct bilirubin in urine.
- This technology offers a promising noninvasive alternative to traditional blood sampling methods.
- The device demonstrates high accuracy and sensitivity for direct bilirubin quantification.
