Saliva Diagnosis Using Small Extracellular Vesicles and Salivaomics
Pingping Han1,2, Xiang Li3,4,5, Wei Wei3,5
1School of Dentistry, The University of Queensland, Brisbane, Australia.
Methods in Molecular Biology (Clifton, N.J.)
|November 23, 2022
Summary
Saliva analysis using small extracellular vesicles (sEVs) and multi-omics shows promise for diagnosing oral diseases like periodontitis. This study details methods for isolating sEVs and performing salivaomics for future dental research.
Area of Science:
- Oral biology and diagnostics
- Biomarker discovery
Background:
- Saliva is a complex biofluid containing diverse molecules and vesicles.
- Oral diseases, such as periodontitis, require novel diagnostic approaches.
- Salivary small extracellular vesicles (sEVs) and multi-omics are emerging biomarkers.
Purpose of the Study:
- To describe methodologies for isolating salivary sEVs.
- To outline procedures for performing salivaomics.
- To guide future dental research in diagnostics.
Main Methods:
- Isolation of salivary small extracellular vesicles (sEVs) via size exclusion chromatography.
- Saliva multi-omics analysis including microbiome, transcriptome, DNA methylome, and proteome.
Main Results:
- Established protocols for salivary sEV isolation and salivaomics.
- Demonstrated the potential of these methods for oral disease research.
Conclusions:
- Salivary sEVs and multi-omics represent promising avenues for oral disease diagnostics.
- The described methodologies can advance dental research and clinical applications.
Related Concept Videos
01:23Salivary Glands and Saliva
829
The salivary glands, of which there are three pairs known as the parotid, submandibular, and sublingual glands, play a crucial role in maintaining oral health and initiating the digestive process. Positioned near the ears, beneath the masseter muscle, the parotid glands secrete saliva into the oral cavity through the parotid duct of Stensen. Meanwhile, the submandibular glands, located on the floor of the mouth, secrete saliva through channels named submandibular ducts. The sublingual glands,...
829
01:36Overview of Exosomes
2.8K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
2.8K