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Updated: Aug 20, 2025
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Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT
Published on: November 27, 2019
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Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps
Josefine Hirschfeld1, Ilaria J Chicca2, Carolyn G J Moonen3
1Department of Periodontology, Birmingham Dental School and Hospital, University of Birmingham, Birmingham, UK. J.Hirschfeld@bham.ac.uk.
Methods in Molecular Biology (Clifton, N.J.)
|November 23, 2022
Summary
Neutrophil extracellular traps (NETs) are crucial in inflammatory diseases like periodontitis. This chapter details methods for isolating neutrophils and analyzing NETs, their antibacterial functions, and clearance mechanisms.
Area of Science:
- Immunology
- Microbiology
- Periodontology
Background:
- Neutrophil extracellular traps (NETs) were discovered in 2004.
- NETs play a significant role in various inflammatory diseases, including periodontitis.
Purpose of the Study:
- To describe current methodologies for neutrophil isolation and NET analysis.
- To explore NETs' role in periodontitis and other inflammatory conditions.
Main Methods:
- Isolation of peripheral blood and oral neutrophils.
- Quantification and visualization of NET production.
- Assessment of NETs' ability to entrap and kill bacteria.
- Evaluation of NET clearance by nuclease-containing plasma.
Main Results:
- Established methods allow for detailed study of NETs.
- NETs demonstrate significant antibacterial activity.
- Nuclease-containing plasma can effectively remove NETs.
Conclusions:
- NETs are a key component in the inflammatory response, particularly in periodontitis.
- Standardized methods are essential for advancing NET research.
- Understanding NET dynamics is vital for therapeutic strategies in inflammatory diseases.
Keywords:
Cathepsin GChemiluminescenceDNAElastaseFluorescence microscopyImmunostainingMyeloperoxidaseNeutrophil extracellular trapsReactive oxygen speciesSEM
