Selective plane illumination microscope dedicated to volumetric imaging in microfluidic chambers.

Area of Science:

• Biomedical Engineering
• Microscopy
• Cell Biology

Background:

• Organ-on-chip technology requires advanced imaging for cellular analysis.
• Existing microscopy techniques may struggle with volumetric imaging within microfluidic devices.

Purpose of the Study:

• To present an original selective plane illumination fluorescence microscope.
• To enable morphological analysis of "Organ-on-chip" biostructures at the cellular scale.

Main Methods:

• A custom-built selective plane illumination fluorescence microscope.
• Microscope designed around microfluidic chips for in-situ 3D imaging.
• Horizontal sample orientation to preserve microfluidic phenomena.

Main Results:

• The setup achieves a field of view of approximately 200 µm with a resolution of approximately 5 µm.
• Demonstrated 3D imaging capabilities within microfluidic chambers.
• Successful imaging of organ-on-chip-like biostructures.

Conclusions:

• The developed microscope provides a viable solution for 3D imaging of organ-on-chip models.
• The simple, integrated design facilitates imaging without disturbing microfluidic processes.
• Enables detailed morphological analysis of cellular structures in microfluidic environments.