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Updated: Jun 30, 2026

Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors

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Published on: May 21, 2008

ChIP-Seq Protocol for In Vitro Cell Differentiation Systems.

Jacqueline Copeland¹, Fjodor Merkuri¹, Marcos Simoes-Costa²

¹Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)

|November 25, 2022

Summary

This summary is machine-generated.

This study details a protocol for ChIP-seq library preparation from human-induced neural crest cells. This method helps identify transcription factor interactions and uncover gene regulatory principles in development.

Keywords:

Chromatin immunoprecipitation sequencing (ChIP-seq)In vitro differentiation Protein–DNA interactions

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Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

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Published on: May 16, 2020

Area of Science:

Developmental Biology
Stem Cell Biology
Genomics

Background:

In vitro differentiation systems are crucial for studying complex biological processes.
Understanding human neural crest cell development is key to developmental biology.
Identifying gene regulatory mechanisms is essential for developmental insights.

Purpose of the Study:

To provide a comprehensive protocol for ChIP-seq library preparation and analysis.
To enable the study of human-induced neural crest cells (hiNCCs) derived from human embryonic stem cells (hESCs).
To facilitate the identification of transcription factor binding sites and cis-regulatory elements.

Main Methods:

Protocol development for ChIP-seq library preparation.
Utilizing human embryonic stem cells (hESCs) for differentiation into hiNCCs.
Analysis of ChIP-seq data to identify protein-DNA interactions.

Main Results:

A detailed and reproducible protocol for ChIP-seq library preparation from hiNCCs.
Successful identification of interactions between transcription factors and cis-regulatory elements.
Establishment of a workflow for uncovering gene regulatory principles.

Conclusions:

The presented protocol is a valuable tool for studying hiNCC development.
This method aids in understanding the molecular basis of gene regulation during development.
The workflow supports future research into developmental disorders and regenerative medicine.