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Updated: Jun 30, 2026

Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors
Published on: May 21, 2008
ChIP-Seq Protocol for In Vitro Cell Differentiation Systems
Jacqueline Copeland1, Fjodor Merkuri1, Marcos Simoes-Costa2
1Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA.
Abstract:
In vitro differentiation systems provide a flexible platform for understanding complex developmental processes. Here, we provide a comprehensive protocol for the preparation and analysis of ChIP-seq libraries for human-induced neural crest cells (hiNCCs) from human embryonic stem cells (hESCs). This workflow is aimed at identifying interactions between transcription factors and cis regulatory elements, which serve as useful assays in uncovering gene regulatory principles during development.

