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Easy NanoString nCounter data analysis with the NanoTube
Caleb A Class1, Caiden J Lukan1, Christopher A Bristow2
1Department of Pharmaceutical Sciences, Butler University, Indianapolis, IN 46208, USA.
Summary:
The NanoTube is an open-source pipeline that simplifies the processing, quality control, normalization and analysis of NanoString nCounter gene expression data. It is implemented in an extensible R library, which performs a variety of gene expression analysis techniques and contains additional functions for integration with other R libraries performing advanced NanoString analysis techniques. Additionally, the NanoTube web application is available as a simple tool for researchers without programming expertise.
Availability And Implementation:
The NanoTube R package is available on Bioconductor under the GPL-3 license (https://www.bioconductor.org/packages/NanoTube/). The R-Shiny application can be downloaded at https://github.com/calebclass/Shiny-NanoTube, or a simplified version of this application can be run on all major browsers, at https://research.butler.edu/nanotube/.
Supplementary Information:
Supplementary data are available at Bioinformatics online.
