Multiscale Approach to Studying Biomolecular Interactions in Cellulose-Casein Adhesion

Area of Science:

• Protein chemistry
• Materials science
• Adhesion science

Background:

• Casein is an eco-friendly adhesive for various materials.
• Protein conformational changes can impact adhesive properties.
• Understanding casein's multiscale behavior is key for optimizing its use.

Purpose of the Study:

• To investigate the multiscale contributions of casein's structural changes to its adhesion.
• To correlate molecular and microstructural changes with macroscopic adhesion performance.
• To identify factors influencing casein-cellulose pressboard bonding.

Main Methods:

• Lap shear strength testing
• Fourier transform infrared spectroscopy (FTIR)
• Microscopy and rheological studies

Main Results:

• Lap shear strength increased with casein concentration.
• α-helical conformations during preconcentration increased hydrophobicity and affected wetting.
• Casein compositions lacking α-helices enhanced bonding strength.

Conclusions:

• Adhesion involves multiscale interactions: molecular, conformational, microstructural, and substrate porosity.
• Tuning casein's structure and dispersion properties can optimize adhesive applications.
• Casein's adhesion to cellulose is influenced by protein conformation and substrate characteristics.