A Review on Spike and Recovery Method in Analytical Method Development and Validation

Area of Science:

• Forensic Science
• Analytical Chemistry
• Toxicology

Background:

• Analytical approaches are vital in multidisciplinary science.
• Spike and recovery (SAR) is a key technique for assessing analytical method accuracy.
• SAR methods are fundamental in analytical testing.

Purpose of the Study:

• To clarify the role and utility of SAR methods in forensic science.
• To discuss the applications and current role of SAR in Forensic Toxicology.
• To highlight SAR as a critical parameter in analytical method validation.

Main Methods:

• A comprehensive literature search was conducted across multiple scientific databases (Google, Web of Sciences, Scopus, PubMed, Google Scholar, SciFinder).
• Scientific reports on SAR utility were critically explored from sources like Science Direct.
• The review focuses on SAR applications within Forensic Toxicology.

Main Results:

• SAR methodology is essential for the accurate identification and quantitation of analytes in sample matrices.
• SAR is a major parameter in the validation of analytical methods.
• SAR-based studies are fundamental for method development, optimization, quality control, and assurance.

Conclusions:

• SAR methods are indispensable for ensuring quality assurance across scientific disciplines.
• A better understanding and adherence to standard guidelines are needed for SAR utilization.
• SAR plays a substantial role in analytical testing and forensic science applications.