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Case report: Whole genome sequence of Clostridium perfringens JUM001 causing acute emphysematous cholecystitis

Mari Tohya1,2, Tomohiro Otsuka3, Jiro Yoshimoto3

  • 1Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan.

Frontiers in Microbiology
|December 5, 2022
PubMed

Insights

This study identified Clostridium perfringens JUM001 as the cause of acute emphysematous cholecystitis. Whole-genome sequencing revealed its genetic makeup, including toxin types and plasmid families, offering insights into this severe infection.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Acute emphysematous cholecystitis is a severe gallbladder inflammation.
  • Clostridium perfringens is an opportunistic pathogen.
  • Understanding the genetic basis of C. perfringens infections is crucial.

Observation:

  • A patient with emphysematous cholecystitis had bile microbiota dominated by Clostridium perfringens (99.73%).
  • The isolated strain, C. perfringens JUM001, was identified as the causative agent.
  • Metagenomic and complete genome sequencing were performed on the bile sample.

Findings:

  • C. perfringens JUM001 possesses a chromosome and two plasmids (pJUM001-1, pJUM001-2).
  • The strain is Toxinotype A, identified by the presence of the plc gene.
  • Phylogenetic analysis indicates a close relationship with a canine C. perfringens strain.

Implications:

  • This is the first whole-genome report of a C. perfringens clinical isolate causing acute emphysematous cholecystitis.
  • The genomic data provides a foundation for understanding the virulence factors and evolution of C. perfringens.
  • Further research can explore therapeutic strategies targeting specific genomic features of this pathogen.
Keywords:
Clostridium perfringensemphysematous cholecystitispCP13-like family plasmidpCW3-like family plasmidtoxinotype Awhole-genome sequencingα-toxin

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