Related Experiment Videos
Acid- and cryoactivation of renin in human plasma
Klinische Wochenschrift
|May 3, 1979
Summary
This study demonstrates that both cryoactivation and acid-activation effectively convert inactive renin to active renin in human plasma. The results suggest these optimized methods fully activate renin, validating the term "total renin".
Area of Science:
- Biochemistry
- Physiology
Background:
- Inactive renin circulates in human plasma and requires activation to become enzymatically active.
- Understanding the complete activation of inactive renin is crucial for accurate renin-based diagnostics and research.
Purpose of the Study:
- To compare the efficacy of cryoactivation and acid-activation in converting inactive renin to active renin.
- To determine if optimized activation procedures can fully convert all inactive renin present in plasma.
Main Methods:
- Human plasma samples were subjected to two distinct in vitro activation protocols: cryoactivation (0°C/-5°C for up to 3 months) and acid-activation (dialysis to pH 3.0 for 48 hours, then pH 7.5).
- Plasma renin concentration was measured after each activation procedure.
Main Results:
- Both cryoactivation and acid-activation yielded similar plasma renin concentrations (96 ± 50 microU/ml vs 100 ± 43 microU/ml).
- No significant increase in acid-activable renin was observed after cryoactivation, indicating complete conversion.
Conclusions:
- Optimized cryoactivation and acid-activation procedures are effective in completely converting inactive renin to active renin in human plasma.
- The term "total renin" is considered valid under these complete activation conditions.