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Related Experiment Video

Updated: Aug 17, 2025

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

Published on: June 2, 2018

Generation of Insulin-Producing Multicellular Organoids.

Laura Mar Fonseca^1,2,3, Fanny Lebreton^1,2,3, Charles-Henri Wassmer⁴

¹Laboratory of Tissue Engineering and Organ Regeneration, Department of Surgery, University of Geneva, Geneva, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)

|December 12, 2022

Summary

This summary is machine-generated.

This study details methods for creating insulin-secreting organoids, offering a promising alternative to clinical islet transplantation (CIT) for type 1 diabetes. These organoids aim to overcome CIT

Keywords:

Amniotic epithelial cells Diabetes EndoC-BH1 Insulin-secreting Islets Multicellular Organoids

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Last Updated: Aug 17, 2025

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Published on: June 2, 2018

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Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters

Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters

Published on: June 23, 2023

Area of Science:

Regenerative Medicine
Endocrinology
Transplantation Biology

Background:

Clinical islet transplantation (CIT) for type 1 diabetes (T1D) improves glycemic control but faces limitations like donor scarcity, lifelong immunosuppression, and graft failure due to inflammation and ischemia.
Organoid generation is a promising regenerative medicine approach to address CIT limitations by enabling scalable production and improved graft survival.

Purpose of the Study:

To detail a stepwise methodology for generating insulin-secreting organoids using two distinct methods.
To describe in vitro quality assessment tests for these novel beta-cell replacement therapies.

Main Methods:

Generation of multicellular insulin-secreting organoids using islet cells and human amniotic epithelial cells (hAECs).
Detailed step-by-step protocols for organoid fabrication.
In vitro quality control assays for assessing organoid viability and function.

Main Results:

Successfully generated 3D insulin-secreting organoid structures.
Demonstrated improved viability and function of these organoids in vitro.
Previous in vivo studies showed enhanced graft survival and function.

Conclusions:

The described methodology provides a reproducible approach for generating insulin-secreting organoids.
These organoids represent a potential advancement over current clinical islet transplantation for T1D treatment.
Further research can optimize organoid generation for enhanced therapeutic efficacy and reduced immune rejection.