Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

15-LOX-catalytic bias towards ether-(alkenyl)-ETE-PEs oxidation bestows selectivity of PRO-ferroptotic cell death signaling.

Nature communications·2026
Same author

Corrigendum to: Mitochondrial bioenergetics and cardiolipin remodeling abnormalities in mitochondrial trifunctional protein deficiency.

JCI insight·2026
Same author

Leveraging Expert Knowledge and Causal Structure Learning to Build Parsimonious Models of Acute Brain Dysfunction in the Pediatric Intensive Care Unit (PICU).

medRxiv : the preprint server for health sciences·2026
Same author

Differential effects of the microbial metabolite acetate on murine microglia in in vitro sepsis and trauma models.

Brain research·2026
Same author

A new sesquiterpenoid from the Fungus Aspergillus sp. and its insecticidal activity.

Biochemical and biophysical research communications·2026
Same author

Microbial production of short-chain fatty acids attenuates long-term neurologic impairment after traumatic brain injury.

Journal of neuroinflammation·2025
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Aug 17, 2025

Time-Resolved In Vivo Measurement of Neuropeptide Dynamics by Capacitive Immunoprobe in Porcine Heart
08:20

Time-Resolved In Vivo Measurement of Neuropeptide Dynamics by Capacitive Immunoprobe in Porcine Heart

Published on: May 19, 2022

2.1K

Detecting and Quantifying pADPr In Vivo.

Andrew M Lamade1, Yaming Chen1, Carla J Johnson1

  • 1Safar Center for Resuscitation Research, Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 14, 2022
PubMed
Summary
This summary is machine-generated.

Monitoring poly(ADP-ribose) polymerases (PARP) activation by quantifying poly-ADP-ribosylation (pADPr) offers insights into disease and aids PARP inhibitor therapy. Detecting pADPr accumulation is crucial for understanding PARP

Keywords:
ADP-ribose polymerasePoly(ADP-ribose) polymerasePoly(ADP-ribose) polymersPoly(ADP-ribose) synthetasePoly-ADP-ribosylation

More Related Videos

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog
08:37

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog

Published on: November 5, 2014

10.0K
Microfabricated Post-Array-Detectors mPADs: an Approach to Isolate Mechanical Forces
61:34

Microfabricated Post-Array-Detectors mPADs: an Approach to Isolate Mechanical Forces

Published on: October 1, 2007

12.6K

Related Experiment Videos

Last Updated: Aug 17, 2025

Time-Resolved In Vivo Measurement of Neuropeptide Dynamics by Capacitive Immunoprobe in Porcine Heart
08:20

Time-Resolved In Vivo Measurement of Neuropeptide Dynamics by Capacitive Immunoprobe in Porcine Heart

Published on: May 19, 2022

2.1K
Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog
08:37

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog

Published on: November 5, 2014

10.0K
Microfabricated Post-Array-Detectors mPADs: an Approach to Isolate Mechanical Forces
61:34

Microfabricated Post-Array-Detectors mPADs: an Approach to Isolate Mechanical Forces

Published on: October 1, 2007

12.6K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cellular Biology

Background:

  • Poly(ADP-ribose) polymerases (PARP) are key enzymes in DNA repair and cellular homeostasis.
  • PARP overactivation leads to NAD+ depletion, mitochondrial dysfunction, and cell death, contributing to various diseases.
  • PARP inhibitors are effective in cancer therapy, highlighting the importance of monitoring PARP activity.

Purpose of the Study:

  • To discuss non-isotopic immunodetection methods for quantifying poly-ADP-ribosylation (pADPr).
  • To emphasize the significance of monitoring PARP-1 activation in disease and therapeutic drug monitoring.

Main Methods:

  • Western blotting for poly-ADP-ribosylated proteins.
  • Immunohistochemistry for cellular localization of pADPr.
  • Enzyme-linked immunoassay (ELISA) for pADPr quantification.
  • Two-dimensional gel electrophoresis for pADPr analysis.

Main Results:

  • Several non-isotopic immunodetection methods can effectively quantify pADPr.
  • These methods allow for the detection and quantification of PARP activation.
  • Monitoring pADPr provides mechanistic insights and aids in therapeutic drug monitoring for PARP inhibitors.

Conclusions:

  • Quantifying pADPr is essential for understanding PARP's role in disease.
  • Non-isotopic immunodetection methods offer valuable tools for assessing PARP activity.
  • Monitoring pADPr levels can guide PARP inhibitor therapy and improve patient outcomes.