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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Related Experiment Video

Updated: Aug 17, 2025

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

BID-seq: The Quantitative and Base-Resolution Sequencing Method for RNA Pseudouridine.

Li-Sheng Zhang¹, Qing Dai¹, Chuan He¹

¹Department of Chemistry and the Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois 60637, United States.

ACS Chemical Biology

|December 16, 2022

Summary

This summary is machine-generated.

Bisulfite-Induced Deletion sequencing (BID-seq) quantitatively detects mRNA pseudouridine modifications at base resolution. This advancement is crucial for understanding RNA modifications and future clinical applications.

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2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

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Area of Science:

Molecular Biology
Genomics
Biochemistry

Background:

Quantitative and base-resolution sequencing are essential for studying RNA modifications.
Diverse RNA modifications play critical roles in biological functions.
Current methods are vital for both fundamental research and future clinical applications.

Discussion:

This article introduces Bisulfite-Induced Deletion sequencing (BID-seq).
BID-seq enables quantitative detection of mRNA pseudouridine (Ψ) modifications.
The method achieves base-resolution accuracy.

Key Insights:

BID-seq provides a novel approach for precise RNA modification analysis.
The technique allows for quantitative assessment of pseudouridine levels.
Base-resolution detection is key to understanding modification impacts.

Outlook:

BID-seq holds potential for future clinical studies and applications.
Advancements in sequencing methods accelerate RNA biology research.
Further development could enhance diagnostic and therapeutic strategies.