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Updated: Aug 17, 2025

Accessing the Cytotoxicity and Cell Response to Biomaterials
Published on: July 8, 2021
Cell viability and physicochemical effects of different concentrations of bismuth trioxide in a mineral trioxide
Karla Padrón-Alvarado1, Laura A García-Mendoza1, Juana P Ramírez-Ortega1
1Dental Materials and Biomaterials Research Laboratory, Postgraduate Studies and Research Division, Faculty of Dentistry, National Autonomous University of México.
Purpose:
To evaluate the effect of three concentrations of bismuth trioxide (Bi2O3) on the biological and physicochemical properties of an experimental mineral trioxide aggregate-type (MTA-type) cement at different time points.
Methods:
Three experimental groups with white Portland cement containing 15, 20, or 25 wt% of Bi2O3 were assessed. Cellular proliferation in human periodontal ligament fibroblasts was evaluated with an MTT assay. Radiopacity, dimensional stability, pH, and compressive strength were evaluated at different time points.
Results:
Bismuth trioxide induced cell proliferation in the Bi15 and Bi25 groups in a time-dependent manner; pH was similar in all groups. Compressive strength was associated with time and bismuth concentration. Bi25 had significantly contracted at day 7 and expanded at day 14 (ANOVA P < 0.05, post hoc Tukey test P < 0.05).
Conclusion:
A higher Bi2O3 concentration had a negative effect on the physical properties of the cement at all time points.
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