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Related Experiment Video

Updated: Aug 16, 2025

Laser Microdissection for Species-Agnostic Single-Tissue Applications
08:57

Laser Microdissection for Species-Agnostic Single-Tissue Applications

Published on: March 31, 2022

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Tissue harvest with a laser microbiopsy.

Jason B King1, Nitesh Katta2, Sapun H Parekh1

  • 1The University of Texas at Austin, Department of Biomedical Engineering, Austin, Texas, United States.

Journal of Biomedical Optics
|December 19, 2022
PubMed
Summary
This summary is machine-generated.

A novel laser microbiopsy device enables minimally invasive tissue sampling. This technique, combined with virtual hematoxylin and eosin (H&E) imaging, offers a promising approach for rapid, accurate tissue diagnosis.

Keywords:
laser ablationmicrobiopsyminimally invasivevirtual hematoxylin and eosin

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Area of Science:

  • Biomedical Engineering
  • Pathology
  • Laser Technology

Background:

  • Traditional pathology workflows face challenges with invasiveness, limited sample analysis, and lengthy processing times.
  • Current methods often analyze only a small portion of large tissue samples, impacting diagnostic accuracy.
  • Minimally invasive biopsy techniques are crucial for improving patient outcomes and diagnostic efficiency.

Purpose of the Study:

  • To develop a laser microbiopsy device for minimally invasive harvest of sub-microliter tissue volumes.
  • To integrate laser microbiopsy with virtual hematoxylin and eosin (H&E) imaging for rapid tissue diagnosis.
  • To evaluate the efficacy and safety of the laser microbiopsy technique in preclinical models.

Main Methods:

  • Utilized an annular Ho:YAG laser beam to ablate tissue, ejecting a central core for collection onto a glass slide.
  • Employed cryogen spray cooling before and after laser ablation to minimize thermal damage to the tissue.
  • Harvested microbiopsies from porcine skin and kidney, followed by confocal microscopy and digital false coloring for virtual H&E imaging.

Main Results:

  • Successfully harvested microbiopsies from porcine skin and kidney tissues.
  • Demonstrated that cryogen cooling effectively limits thermal damage during laser microbiopsy.
  • Virtual H&E images of the harvested microbiopsies preserved key cellular features, including cell nuclei.

Conclusions:

  • Laser microbiopsy coupled with virtual H&E imaging presents a promising advancement in biopsy and diagnostic procedures.
  • The technique offers a rapid and minimally invasive alternative to conventional pathology methods.
  • Further research may establish laser microbiopsy as a valuable tool in clinical diagnostics.