Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

AND-Logic-Gated Aptamer Switch for Precise Targeting and Regulation of RNA G-Quadruplexes.

Angewandte Chemie (International ed. in English)·2026

Same author

Recent Advances in Chemical Probing Strategies for RNA Structure Determination In Vivo.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026

Same author

MoRNiNG: A Database of RNA Modification Sites Associated with RNA Secondary Structure Dynamics.

Genomics, proteomics & bioinformatics·2025

Same author

L-RNA Aptamer-Based Tools for G-Quadruplex Structure: Identification, Characterization, and Application.

Accounts of chemical research·2025

Same author

Differential Evolution of CDS and UTR Non-canonical RNA G-quadruplex Structures in Eukaryotic Transcriptomes.

Genomics, proteomics & bioinformatics·2025

Same author

TDP-43 binds to RNA G-quadruplex structure and regulates mRNA stability and translation.

Nucleic acids research·2025

Search research articles

Related Experiment Video

Updated: Aug 16, 2025

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Capture-SELEX: Selection Strategy, Aptamer Identification, and Biosensing Application.

Sin Yu Lam¹, Hill Lam Lau¹, Chun Kit Kwok^1,2

¹State Key Laboratory of Marine Pollution and Department of Chemistry, City University of Hong Kong, Hong Kong SAR 999077, China.

|December 23, 2022

Summary

This summary is machine-generated.

Developing aptasensors for detecting harmful small-molecule contaminants is crucial. Capture-SELEX is a key method for creating aptamers used in these biosensors, offering a promising future for environmental and health monitoring.

Keywords:

DNA and RNA Capture-SELEX aptamer biosensing applications characterization nucleic acid aptamer small molecule contaminant

More Related Videos

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Related Experiment Videos

Last Updated: Aug 16, 2025

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Area of Science:

Environmental Science
Biotechnology
Analytical Chemistry

Background:

Small-molecule contaminants like antibiotics, pesticides, and plasticizers pose significant risks to human health and ecosystems.
Traditional biosensors often rely on antibodies, but aptamers offer a promising alternative for contaminant detection.

Purpose of the Study:

To review recent strategies for Capture-SELEX, a method for generating aptamers against small molecules.
To discuss methods for characterizing aptamer-small molecule interactions.
To explore the development and applications of aptamer-based biosensors (aptasensors).

Main Methods:

Systematic Evolution of Ligands by Exponential Enrichment (SELEX), specifically the Capture-SELEX modification.
Characterization techniques for aptamer-target binding.
Development of aptasensor platforms for contaminant detection.

Main Results:

Capture-SELEX has become a widely adopted method for generating DNA/RNA aptamers targeting small molecules.
Various methods exist for detecting and characterizing these specific molecular interactions.
Aptasensors show potential for diverse applications in contaminant monitoring.

Conclusions:

Aptasensors offer a low-cost, portable solution for rapid detection of harmful small-molecule contaminants.
Capture-SELEX is a powerful tool for aptamer generation, driving advancements in biosensor technology.
Further research into Capture-SELEX and aptasensor development is needed to overcome current challenges and realize future applications.