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Crystallization of a DNA duplex 15-mer containing unpaired bases: d(CGCGAAATTTACGCG)
M Miller1, A Wlodawer, E Appella
1Center for Chemical Physics, National Bureau of Standards, Gaithersburg, MD 20899.
Journal of Molecular Biology
|June 20, 1987
Abstract:
Crystals of an almost self-complementary DNA 15-mer d(CGCGAAATTTACGCG) have been grown by the vapor diffusion technique at 4 degrees C. The space group is I222 with a = 37.3 A, b = 54.6 A and c = 104.8 A. Solution studies showed that the 15-mer forms a duplex with the extra adenine residue unpaired: (sequence; see text) Crystals are stable at 4 degrees C and are suitable for medium-resolution structural studies.