Process Technologies of Cyanobacteria

Area of Science:

• Biotechnology
• Microbiology
• Phycology

Background:

• Cyanobacteria present significant challenges in handling and exploitation.
• These phototrophic bacteria hold immense potential for innovative biotechnological applications.

Purpose of the Study:

• To provide a comprehensive overview of working with cyanobacteria.
• To cover screening methods, conservation techniques, vitality assessment, and cultivation strategies.
• To present novel findings on pre-culture influence and photobioreactor designs.

Main Methods:

• In silico and in vitro screening for bioactive substances.
• Comparison of conservation techniques and vitality/viability estimation methods.
• Non-invasive vitality evaluation using pulse amplitude modulated fluorometry.
• Investigation of pre-culture state influence on main cultures.
• Analysis of various photobioreactor designs, including biofilm systems.
• Exploration of heterotrophic and mixotrophic cultivation modes.

Main Results:

• Established up-to-date screening and conservation methods.
• Demonstrated non-invasive vitality evaluation via pulse amplitude modulated fluorometry.
• Revealed novel insights into the impact of pre-culture conditions on cyanobacterial growth.
• Compared different photobioreactor designs, highlighting biofilm systems.
• Summarized literature and presented data on enhanced cyanobacterial production through heterotrophic and mixotrophic cultivation.

Conclusions:

• Cyanobacteria offer versatile biotechnological opportunities.
• Optimized methods for screening, conservation, and cultivation are crucial for harnessing their potential.
• Novel approaches in photobioreactor design and cultivation modes can significantly enhance production processes.