Protection of Skin Fibroblasts from Infrared-A-Induced Photo-Damage Using Ginsenoside Rg3(S)-Incorporated Soybean...

Area of Science:

• Dermatology
• Biotechnology
• Cosmetic Science

Background:

• Chronic infrared-A (IRA) irradiation contributes to skin photoaging.
• Protecting skin cells from IRA damage is essential for anti-aging strategies.

Purpose of the Study:

• To evaluate the photoprotective effects of Rg3(S) and Rg3(S)-incorporated anionic soybean lecithin liposomes (Rg3/Lipo) against IRA-induced photodamage.
• To assess the solubility, stability, and biocompatibility of the Rg3/Lipo formulation.

Main Methods:

• Formulation of Rg3/Lipo with an approximate size of 150 nm.
• Assessment of Rg3/Lipo aqueous solubility and colloidal stability.
• Evaluation of Rg3/Lipo biocompatibility with normal human dermal fibroblasts (NHDFs).
• Exposure of NHDFs to repeated IRA irradiation and measurement of cellular/mitochondrial reactive oxygen species (ROS) and apoptotic signals.

Main Results:

• Rg3/Lipo demonstrated enhanced aqueous solubility and superior colloidal stability compared to free Rg3(S).
• Rg3/Lipo exhibited good biocompatibility with NHDFs.
• Both Rg3(S) and Rg3/Lipo significantly reduced IRA-induced cellular and mitochondrial ROS levels.
• Rg3/Lipo treatment notably decreased apoptotic signals in IRA-irradiated NHDFs.

Conclusions:

• Rg3/Lipo presents improved physicochemical properties, including solubility and stability.
• Rg3/Lipo effectively mitigates IRA-induced oxidative stress and apoptosis in skin fibroblasts.
• Rg3/Lipo is a promising candidate for infrared protection, supporting healthy skin aging.