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Updated: Aug 17, 2026

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
Published on: April 17, 2017
Concentration dependence of the binding constant of antibodies
1Department of Microbiology, State University of New York, Buffalo 14214.
Abstract:
By simultaneous dilution of the antigen (AG) and the antibody (AB) used in an immune precipitation reaction, at the equivalence AG/AB ratio, it was found that the association constant, Ka, increased from 1.7 X 10(7) 1/M at the highest concn of AG and AB, to 6.5 X 10(11) 1/M at 100-fold dilution of both. An important (but not the only) reason for this increase is taken to be the fact that the AB solution was polyclonal.
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