Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

DNAi: an open-source AI tool for unbiased DNA fiber analysis.

Nucleic acids research·2026

Same author

A new approach for high-content traction force microscopy to characterize large cell ensembles.

Biophysical journal·2025

Same author

Characterization of functional and structural impairments in best vitelliform macular dystrophy using visual electrophysiology and optical coherence tomography in pediatric and adult patients.

Documenta ophthalmologica. Advances in ophthalmology·2025

Same author

R-2-hydroxyglutarate-mediated inhibition of KDM4A compromises telomere integrity.

Nucleic acids research·2025

Same author

Spatial and Temporal Changes in Choroid Morphology Associated With Long-Duration Spaceflight.

Investigative ophthalmology & visual science·2025

Same author

Focus on numbers - characterizing protein accumulation at DNA double-strand breaks.

Journal of cell science·2025

Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Aug 15, 2025

RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling

RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling

Published on: April 11, 2015

Laser-Assisted Single-Cell Labeling and Capture.

Nicolas Desjardins-Lecavalier¹, Graziana Modica¹, Santiago Costantino^2,3

¹Maisonneuve-Rosemont Hospital Research Center, Montreal, QC, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|December 31, 2022

Summary

This summary is machine-generated.

New cell labeling via photobleaching (CLaP) and single-cell magneto-optical capture (scMOCa) techniques enable researchers to isolate specific live cells from complex mixtures without genetic modification. These cost-effective methods support transcriptomic and biological characterization of captured cells.

Keywords:

CLaP Laser scanning microscope Photobleaching Single-cell capture Single-cell tag scMOCa

More Related Videos

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

Published on: April 10, 2013

Fluorescence-Guided Matrix-assisted Laser Desorption/Ionization with Laser-Induced Postionization Mass Spectrometry of Individual Rat Neural Cells

Fluorescence-Guided Matrix-assisted Laser Desorption/Ionization with Laser-Induced Postionization Mass Spectrometry of Individual Rat Neural Cells

Published on: May 23, 2025

Related Experiment Videos

Last Updated: Aug 15, 2025

RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling

RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling

Published on: April 11, 2015

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

Published on: April 10, 2013

Fluorescence-Guided Matrix-assisted Laser Desorption/Ionization with Laser-Induced Postionization Mass Spectrometry of Individual Rat Neural Cells

Fluorescence-Guided Matrix-assisted Laser Desorption/Ionization with Laser-Induced Postionization Mass Spectrometry of Individual Rat Neural Cells

Published on: May 23, 2025

Area of Science:

Cell biology
Biotechnology
Cancer research

Background:

Single-cell technologies are vital for understanding biological systems, including cancer heterogeneity.
Current methods for isolating cells often require genetic manipulation and fluorescent probes.
Distinguishing cells based solely on fluorescence requires a deep understanding of molecular interactions and phenotypes.

Purpose of the Study:

To introduce and detail protocols for Cell Labeling via Photobleaching (CLaP) and single-cell magneto-optical capture (scMOCa).
To provide researchers with cost-effective, non-invasive methods for isolating specific live cells from heterogeneous populations.
To enable subsequent transcriptomic and biological characterization of captured single cells.

Main Methods:

Cell Labeling via Photobleaching (CLaP) utilizes laser-based photobleaching for cell identification.
Single-cell magneto-optical capture (scMOCa) employs magnetic principles for cell isolation.
Both techniques are implemented using standard confocal microscopy lasers and commercially available reagents.

Main Results:

CLaP and scMOCa allow for the selection and capture of individual cells based on observed phenotypes from imaging.
These methods do not alter cellular physiology, preserving cells for downstream analyses.
The protocols are designed to be convenient, cost-effective, and require minimal specialized equipment.

Conclusions:

CLaP and scMOCa offer powerful, accessible tools for single-cell isolation and analysis.
These techniques overcome limitations of traditional methods by avoiding genetic modification.
The described protocols facilitate advanced research in developmental biology, cancer biology, and beyond.