Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.1K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

5.0K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
5.0K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.5K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Editorial: Evolving economies in sports: management practices and market impacts.

Frontiers in sports and active living·2026
Same author

Bias Alleviation Through Network Pruning for Sparse and Debiased Models.

IEEE transactions on image processing : a publication of the IEEE Signal Processing Society·2026
Same author

Therapeutic targeting of blood-derived protein infiltration to modulate neuroinflammation in cerebellar ataxia.

Journal of neuroinflammation·2026
Same author

Metabotropic glutamate receptor 5 expression associates with pain and inflammatory pathways in interstitial cystitis.

Scientific reports·2026
Same author

Tannic acid-loaded mesoporous silica particles as tissue adhesives for enhanced wound healing.

Biomaterials science·2026
Same author

A Selective Deposition Strategy of Ultrathin Metal Layer on Sub-Micrometer-Pitch Cu Interconnection for Low-Temperature Hybrid Bonding.

Small science·2026
Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026
Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026
Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026
Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026
Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026
Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026
See all related articles

Related Experiment Video

Updated: Aug 15, 2025

Smartphone Fundus Photography
05:51

Smartphone Fundus Photography

Published on: July 6, 2017

39.2K

Smartphone-based single snapshot spatial frequency domain imaging.

Jesse H Lam1, Kelsey J Tu2, Jeonghun Kim2,3

  • 1Dankook University, Beckman Laser Institute Korea, School of Medicine, Cheonan, Chungnam, Republic of Korea.

Biomedical Optics Express
|January 2, 2023
PubMed
Summary
This summary is machine-generated.

We developed a smartphone-based spatial frequency domain imaging (SFDI) device for accessible skin analysis. This novel handheld imaging system accurately measures optical properties and hemodynamics, paving the way for cosmetic and dermatological applications.

More Related Videos

Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System: Applications to Cell Adhesion
10:30

Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System: Applications to Cell Adhesion

Published on: September 4, 2013

9.7K
Dual Raster-Scanning Photoacoustic Small-Animal Imager for Vascular Visualization
07:14

Dual Raster-Scanning Photoacoustic Small-Animal Imager for Vascular Visualization

Published on: July 15, 2020

4.2K

Related Experiment Videos

Last Updated: Aug 15, 2025

Smartphone Fundus Photography
05:51

Smartphone Fundus Photography

Published on: July 6, 2017

39.2K
Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System: Applications to Cell Adhesion
10:30

Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System: Applications to Cell Adhesion

Published on: September 4, 2013

9.7K
Dual Raster-Scanning Photoacoustic Small-Animal Imager for Vascular Visualization
07:14

Dual Raster-Scanning Photoacoustic Small-Animal Imager for Vascular Visualization

Published on: July 15, 2020

4.2K

Area of Science:

  • Biomedical optics
  • Medical imaging
  • Optical diagnostics

Background:

  • Spatial Frequency Domain Imaging (SFDI) is a powerful technique for non-invasively quantifying tissue optical properties.
  • Existing SFDI systems are often bulky, expensive, and require specialized training.
  • There is a need for portable, user-friendly, and cost-effective imaging solutions for widespread clinical and research use.

Purpose of the Study:

  • To develop and validate a handheld, smartphone-based Spatial Frequency Domain Imaging (SFDI) device.
  • To assess the device's performance using liquid phantoms and human tissue.
  • To demonstrate the potential for dermatological and cosmetic applications.

Main Methods:

  • Characterized the dynamic range of a smartphone camera sensor.
  • Quantified optical properties (absorption and scattering) of liquid phantoms with varying concentrations.
  • Calculated optical properties and hemoglobin-based chromophores in human wrist tissue.
  • Recorded hemodynamic changes during arterial occlusion in a human hand.

Main Results:

  • The smartphone-based SFDI device demonstrated a linear dynamic range.
  • Accurate separation of absorption and scattering properties was achieved in phantoms.
  • The device successfully calculated optical properties and chromophores in human tissue.
  • Hemodynamic responses were captured in vivo during induced arterial occlusion.

Conclusions:

  • A novel, handheld, smartphone-based SFDI device has been successfully developed and validated.
  • The device shows promise for accessible, quantitative optical imaging in biological tissues.
  • This technology lays the foundation for affordable SFDI devices for dermatological and cosmetic applications.