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Microdissection and Whole Mount Scanning Electron Microscopy Visualization of Mouse Choroid Plexus
Published on: December 16, 2022
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Microdissection and Whole Mount Scanning Electron Microscopy Visualization of Mouse Choroid Plexus.
Elien Van Wonterghem1, Lien Van Hoecke1, Griet Van Imschoot1
1VIB Center for Inflammation Research, VIB; Department of Biomedical Molecular Biology, Ghent University.
Journal of Visualized Experiments : Jove
|January 2, 2023
Summary
This study presents a simple method to isolate the mouse choroid plexus (CP) for neuroscience research. The technique preserves CP tissue integrity, enabling detailed analysis of this crucial brain structure.
Area of Science:
- Neuroscience
- Cell Biology
- Anatomy
Background:
- The choroid plexus (CP) is an understudied, highly vascularized brain tissue crucial for central nervous system (CNS) health and disease.
- Accurate isolation of the CP is essential for downstream functional and structural analyses.
Purpose of the Study:
- To describe a straightforward method for isolating the lateral and fourth ventricle mouse CP.
- To ensure the isolation technique preserves CP cell viability, function, and structure for further research.
Main Methods:
- Isolation of mouse choroid plexus from lateral and fourth brain ventricles without specialized equipment.
- Visualization of CP using a binocular microscope and bromophenol blue during transcardial perfusion.
- Application of techniques like RNA, protein, single cell analysis, and scanning electron microscopy (SEM) on isolated CP.
Main Results:
- A reproducible method for CP isolation that maintains tissue integrity.
- Demonstration of CP visualization techniques compatible with downstream analyses.
- Successful application of SEM for structural overview of the whole mount CP.
Conclusions:
- The described method provides accessible isolation of viable mouse choroid plexus for comprehensive analysis.
- This technique facilitates deeper understanding of the choroid plexus's role in CNS function and pathology.
