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Related Experiment Videos

Myogenesis in vitro as seen with the scanning electron microscope.

Y Shimada1, M Komiyama, M Shiozaki

  • 1Department of Anatomy, School of Medicine, Chiba University, Japan.

Scanning Microscopy
|September 1, 1987
PubMed
Summary

Scanning electron microscopy (SEM) revealed dynamic changes in chick skeletal muscle cell structures during differentiation. Actin filaments form a dense network, and intracellular organelles like mitochondria and sarcoplasmic reticulum show complex arrangements crucial for myogenesis.

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Area of Science:

  • Cell Biology
  • Developmental Biology
  • Microscopy

Background:

  • Skeletal muscle development involves complex cellular and organelle differentiation.
  • Understanding the dynamic changes in cell surface and cytoplasmic structures is key to myogenesis.

Purpose of the Study:

  • To investigate the differentiation of cell surface and cytoplasmic organelles in embryonic chick skeletal muscle cells using scanning electron microscopy (SEM).
  • To characterize the cytoskeletal organization and intracellular membrane systems during myoblast and myotube formation.

Main Methods:

  • Scanning electron microscopy (SEM) was employed to observe cell surface and organelle morphology.
  • Triton extraction, metal coating, and myosin subfragment-1 decoration were used to analyze cytoskeletal structures.

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  • Freeze-polishing and osmium-maceration techniques visualized intracellular organelles.

    • Main Results:

    • Myoblasts maintained a spindle shape during mitosis, with varying filament network densities under the sarcolemma depending on the cell cycle stage.
    • A dense, actin-rich cytoskeletal network was observed in myotubes and fusion areas.
    • Myofibrils aligned with cellular stress lines, and intracellular organelles like mitochondria, T-system tubules, and sarcoplasmic reticulum exhibited complex, interconnected structures.

    Conclusions:

    • SEM provides valuable insights into the morphological changes during skeletal muscle cell differentiation.
    • The study highlights the dynamic organization of the cytoskeleton and intracellular organelles during myogenesis.
    • Further SEM analysis is warranted to deepen our understanding of muscle development.