A computer-assisted study of quantitative morphological features of C type horizontal cells in retina

Biphasic C type horizontal cells of the retina of crucian carp were intracellularly stained with horseradish peroxidase (HRP) and quantitatively studied with computer-aided three-dimensional reconstruction techniques. Several parameters characterizing their morphology were determined and compared among different subtypes of horizontal cells. The results show that both cell body area and horizontal extension are proper parameters by which different subtypes of biphasic horizontal cells can be clearly classified.