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Related Concept Videos

Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

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The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
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Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
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Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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High-Resolution Mass Spectrometry (HRMS)01:15

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The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
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Novel Hybrid Quadrupole-Multireflecting Time-of-Flight Mass Spectrometry System.

Dale A Cooper-Shepherd1, Jason Wildgoose1, Boris Kozlov1

  • 1Waters Corporation, Stamford Avenue, Altrincham Road, Wilmslow, Cheshire, U.K.SK9 4AX.

Journal of the American Society for Mass Spectrometry
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A new mass spectrometry system offers high resolution and accuracy with fast acquisition speeds. This advanced instrument benefits applications like liquid chromatography-mass spectrometry and mass spectrometry imaging.

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Area of Science:

  • Analytical Chemistry
  • Instrumentation Science

Background:

  • High resolution mass spectrometry is crucial for detailed chemical analysis.
  • Existing high-resolution systems often face trade-offs between resolution, accuracy, and speed.

Purpose of the Study:

  • To introduce a novel mass spectrometry system with enhanced performance.
  • To demonstrate the system's applicability in advanced analytical workflows.

Main Methods:

  • Development of a quadrupole-multireflecting time-of-flight mass analyzer.
  • Incorporation of planar, gridless ion mirrors for fourth-order energy focusing.
  • Integration into liquid chromatography-mass spectrometry and mass spectrometry imaging.

Main Results:

  • Achieved resolving power over 200,000 fwhm.
  • Demonstrated sub-ppm mass accuracy.
  • Maintained high performance at relatively fast acquisition speeds.

Conclusions:

  • The novel mass spectrometry system offers superior resolving power and mass accuracy.
  • Its speed and performance make it advantageous for demanding applications.
  • The system is well-suited for liquid chromatography-mass spectrometry and mass spectrometry imaging.