[Amidation of blood proteins during hyperglycemia in experimental diabetes mellitus]

Rates of amidation of blood serum albumin and hemoglobin was studied in hyperglycemia developed during alloxan diabetes mellitus. Content of glucose in blood of the animals with diabetes exceeded 5.2-fold its level in blood of controls; amount of glycosylated hemoglobin was increased by 41.7%. Concentration of total, readily and hardly hydrolyzed amide groups was decreased in hemoglobin of the impaired animals by 13.1%, 17.6% and 10.4%, respectively, as compared with control group. The degree of blood serum albumin amidation was not altered. Hyperglycemia, accompanied by glycosylation of proteins, was the most probable cause of accelerated deamidation of hemoglobin. Participation of the protein deamidation in pathogenesis of diabetes mellitus is discussed.