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RNA Splicing

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

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Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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Related Experiment Video

Updated: Aug 15, 2025

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Sweet splicing.

Maria Carmo-Fonseca¹

¹Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Av. Professor Egas Moniz, 1649-028 Lisbon, Portugal.

|January 7, 2023

Summary

This summary is machine-generated.

Glucose, a primary energy source, also acts as a non-energetic cue. This study reveals glucose regulates alternative splicing in epidermal differentiation by influencing RNA-binding protein DDX21 interactions.

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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

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Published on: September 15, 2021

Area of Science:

Cell biology
Molecular biology
Biochemistry

Background:

Glucose is primarily known as the main energy source for cellular functions.
Cellular differentiation processes are complex and involve intricate regulatory mechanisms.
Alternative splicing is a key mechanism for generating protein diversity.

Purpose of the Study:

To investigate the non-energetic roles of glucose in cellular processes.
To elucidate the molecular mechanisms by which glucose influences gene expression.
To understand glucose's role in epidermal differentiation and alternative splicing.

Main Methods:

Investigated glucose's role beyond energy metabolism.
Utilized molecular biology techniques to study RNA-binding protein interactions.
Analyzed alternative splicing events during keratinocyte differentiation.

Main Results:

Demonstrated that glucose functions as a biomolecular cue.
Showed glucose regulates alternative splicing during epidermal differentiation.
Identified an association between glucose, RNA-binding protein DDX21, and modified splicing decisions.

Conclusions:

Glucose plays a critical, previously unrecognized role in regulating gene expression through alternative splicing.
This finding expands our understanding of glucose's multifaceted functions in cellular regulation.
The glucose-DDX21 interaction offers a new target for understanding and potentially modulating epidermal differentiation.