Related Experiment Videos
[Determination of urinary oxalate using a Sigma kit]
Hinyokika Kiyo. Acta Urologica Japonica
|May 1, 1987
Summary
The Sigma kit offers a reliable and simple method for measuring urinary oxalate, making it suitable for clinical applications. This assay demonstrates good linearity and reproducibility, correlating well with established methods.
Area of Science:
- Biochemistry
- Clinical Chemistry
Context:
- Urinary oxalate measurement is crucial for diagnosing and managing conditions like kidney stones.
- Existing methods are often complex and not practical for routine clinical use.
Purpose:
- To evaluate the Sigma kit as a simple and clinically applicable method for quantifying urinary oxalate.
Summary:
- The Sigma kit utilizes an enzymatic reaction where oxalate is oxidized to hydrogen peroxide, which then reacts to form a colored dye measured spectrophotometrically.
- The method demonstrated excellent linearity (r=0.998) and reproducibility (within-assay CV 1.5-5.0%, between-assay CV 4.0-11.6%).
- Correlations with established methods were strong (0.922 with modified Hodgkinson and Williams, 0.796 with ion chromatography). Ascorbic acid interference was inhibited by FeCl3.
Impact:
- Provides a validated, user-friendly assay for clinical laboratories.
- Facilitates more accessible and accurate diagnosis and monitoring of oxalate-related metabolic disorders.