Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

2.5K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
2.5K
T Cell Types and Functions01:24

T Cell Types and Functions

1.2K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.2K
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

3.5K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
3.5K
Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

69.6K
Overview
69.6K
Special Features of Adaptive Immunity01:20

Special Features of Adaptive Immunity

972
The adaptive immune system, a crucial component of the overall immune response, offers a highly specialized defense against pathogens. It involves specific cell types and features, enabling it to combat infections effectively and efficiently.
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
972
Eukaryotic Compartmentalization01:46

Eukaryotic Compartmentalization

157.1K
One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal cells...
157.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Case Report: Immune checkpoint inhibitor-associated myocarditis, myositis, and myasthenia gravis overlap syndrome with flow cytometric phenotyping before and after treatment in a patient with urothelial carcinoma.

Frontiers in immunology·2026
Same author

Efficacy and safety of the CD40 ligand inhibitor dapirolizumab pegol in systemic lupus erythematosus (PHOENYCS GO): a randomised, double-blind, placebo-controlled, phase 3 trial.

Lancet (London, England)·2026
Same author

Understanding the Therapeutic Potential of PD-1 Agonism in Inflammatory and Autoimmune Disorders.

BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy·2026
Same author

Patient-Friendly Real-Time Optical Tomographic Imaging System (LOTIS) for Lupus Arthritis.

Biosensors·2026
Same author

Bias due to interval censored outcomes in a study of flare risk after hydroxychloroquine taper/cessation in systemic lupus erythematosus.

Journal of clinical epidemiology·2026
Same author

Inhibitory receptor agonists: Emerging strategies in immune modulation.

The Journal of experimental medicine·2026
Same journal

UCA1 lncRNA regulates γ-globin expression by modulating the miR-148b/BCL11A axis.

Life science alliance·2026
Same journal

Democratized single-cell proteomics resolves cell state heterogeneity in skin tumors.

Life science alliance·2026
Same journal

Cross-tissue dual-omics analysis reveals molecular programs linked to myopia susceptibility and progression.

Life science alliance·2026
Same journal

Conserved HSP60 structure with lineage- and context-specific regulation in cnidarians.

Life science alliance·2026
Same journal

TRK-T3 condensate organization drives growth signaling.

Life science alliance·2026
Same journal

Differential A-to-I editing of SINE B2 RNAs unveils an epitranscriptome response to Aβ neurotoxicity.

Life science alliance·2026
See all related articles

Related Experiment Video

Updated: Aug 14, 2025

Preparation of Bead-supported Lipid Bilayers to Study the Particulate Output of T Cell Immune Synapses
11:06

Preparation of Bead-supported Lipid Bilayers to Study the Particulate Output of T Cell Immune Synapses

Published on: April 1, 2022

4.3K

SLAMF6 compartmentalization enhances T cell functions.

Yevgeniya Gartshteyn1,2, Anca D Askanase3,2, Ruijiang Song2

  • 1Division of Rheumatology, Department of Medicine, Columbia University Medical Center, New York, NY, USA yg2372@cumc.columbia.edu.

Life Science Alliance
|January 9, 2023
PubMed
Summary
This summary is machine-generated.

Signaling lymphocyte activation molecule family member 6 (SLAMF6) enhances T cell activation when clustered with the CD3 complex. Bispecific antibodies targeting SLAMF6 show therapeutic potential for modulating T cell responses.

More Related Videos

Imaging the Human Immunological Synapse
09:37

Imaging the Human Immunological Synapse

Published on: December 26, 2019

14.4K
Real-time Live Imaging of T-cell Signaling Complex Formation
10:31

Real-time Live Imaging of T-cell Signaling Complex Formation

Published on: June 23, 2013

14.0K

Related Experiment Videos

Last Updated: Aug 14, 2025

Preparation of Bead-supported Lipid Bilayers to Study the Particulate Output of T Cell Immune Synapses
11:06

Preparation of Bead-supported Lipid Bilayers to Study the Particulate Output of T Cell Immune Synapses

Published on: April 1, 2022

4.3K
Imaging the Human Immunological Synapse
09:37

Imaging the Human Immunological Synapse

Published on: December 26, 2019

14.4K
Real-time Live Imaging of T-cell Signaling Complex Formation
10:31

Real-time Live Imaging of T-cell Signaling Complex Formation

Published on: June 23, 2013

14.0K

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Signaling lymphocyte activation molecule family member 6 (SLAMF6) is a T cell co-receptor crucial for immune responses.
  • Previous research established SLAMF6 clustering as essential for T cell activation.

Purpose of the Study:

  • To investigate the impact of SLAMF6 cell surface compartmentalization on T cell functions.
  • To evaluate SLAMF6 as a potential therapeutic target by understanding its localization-function relationship.

Main Methods:

  • Biochemical assays
  • Co-culture assays
  • Co-immunoprecipitation analysis
  • Utilized bispecific antibodies (anti-CD3/SLAMF6 and anti-CD45/SLAMF6)

Main Results:

  • T cell activity is significantly enhanced when SLAMF6 colocalizes with the CD3 complex.
  • SLAMF6 interacts with proteins critical for T cell receptor downstream signaling, indicating shared pathways.
  • Bispecific anti-CD3/SLAMF6 antibodies promoted SLAMF6-CD3 clustering and enhanced T cell activation.
  • Anti-CD45/SLAMF6 antibodies, despite inhibiting SLAMF6-TCR clustering via steric hindrance, also enhanced T cell activation.

Conclusions:

  • SLAMF6 bispecific antibodies demonstrate potential in modulating T cell responses.
  • Further research is warranted to explore the therapeutic applications of SLAMF6-targeting antibodies in preclinical tumor models.