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Updated: Aug 14, 2025

An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production

An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production

Published on: March 27, 2017

Activity-driven tissue alignment in proliferating spheroids.

Liam J Ruske¹, Julia M Yeomans¹

¹Rudolf Peierls Centre For Theoretical Physics, University of Oxford, UK. liam.ruske@physics.ox.ac.uk.

|January 10, 2023

Summary

This summary is machine-generated.

Cell proliferation gradients in multicellular spheroids drive tissue flows and alignment. Researchers identified three distinct cell alignment patterns, offering insights into tumor dynamics and control mechanisms.

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A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells

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Published on: January 22, 2021

Area of Science:

Biophysics
Cell Biology
Mathematical Biology

Background:

Multicellular spheroids are model systems for tumor dynamics.
Nutrient gradients in spheroids cause differential cell proliferation.

Purpose of the Study:

To model cell flows and tissue dynamics in multicellular spheroids using continuum theory.
To investigate how proliferation gradients influence cell alignment within spheroids.

Main Methods:

Analytical arguments
Three-dimensional simulations
Continuum theory of active nematic fluids

Main Results:

Proliferation gradients induce flows and activity gradients, aligning cells.
Identified three distinct cell alignment regimes: radial, tangential, and mixed (angular near surface, radial in core).

Conclusions:

Continuum modeling can infer cell dynamics from experimental alignment data.
Findings suggest novel mechanisms for controlling cell alignment in aggregates, impacting tumor properties.