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Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation
Published on: July 25, 2017
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A Unique "Reversed" Migration of Neurons in the Developing Claustrum
Kota Oshima1, Satoshi Yoshinaga1,2, Ayako Kitazawa1,2
1Department of Anatomy, Keio University School of Medicine, Tokyo, 160-8582, Japan.
Summary
Claustrum (CLA) neurons migrate outward then inward during development, a unique directional switch. Reelin signaling is crucial for this process, offering insights into brain development and function.
Area of Science:
- Neuroscience
- Developmental Biology
- Neuroanatomy
Background:
- The claustrum (CLA) is vital for higher brain functions.
- CLA dysfunction is linked to neuropsychological symptoms.
- The developmental origins and neuronal migration of the CLA remain poorly understood.
Purpose of the Study:
- To investigate the developmental process of the claustrum (CLA) in mice.
- To analyze the specific migration patterns of CLA neurons.
- To identify molecular mechanisms, such as Reelin signaling, involved in CLA formation.
Main Methods:
- Utilized FlashTag technology to label and track CLA neurons born at embryonic day (E) 11.5.
- Employed time-lapse imaging of GFP-labeled cells to observe neuronal migration.
- Investigated the role of Reelin signaling in CLA neuronal distribution.
Main Results:
- CLA neurons are primarily generated between E10.5 and E12.5, peaking at E11.5.
- Neurons migrate radially outward, reaching the brain surface by E13.5, and then move inward to the CLA by E14.5.
- Reelin signaling is essential for the correct positioning of CLA neurons.
Conclusions:
- CLA neuronal migration involves a distinct outward-then-inward directional switch, differing from typical neuronal migration patterns.
- This unique migration mechanism may be fundamental to the formation of brain nuclei.
- Understanding CLA development and its molecular underpinnings can illuminate the pathogenesis of psychiatric disorders.
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