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Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
Published on: October 27, 2023
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Translational proteomics and phosphoproteomics: Tissue to extracellular vesicles
Xiaofeng Wu1, Anton B Iliuk2, W Andy Tao3
1Department of Chemistry, Purdue University, West Lafayette, IN, United States.
Advances in Clinical Chemistry
|January 15, 2023
Summary
Extracellular vesicles (EVs) offer a non-invasive method for detecting protein and phosphoprotein biomarkers, overcoming limitations of tissue biopsies. This advancement promises routine clinical application for disease diagnosis and monitoring.
Area of Science:
- Translational and clinical medical sciences
- Biomarker discovery
- Proteomics and phosphoproteomics
Background:
- Nucleic acid analysis advances disease understanding but misses phenotypic information.
- Protein phosphorylation regulates cellular functions and provides real-time disease insights.
- Tissue biopsies are invasive and have limitations in protein preservation and heterogeneity.
Purpose of the Study:
- To highlight extracellular vesicles (EVs) as a promising source for non-invasive protein and phosphoprotein biomarker analysis.
- To discuss the advantages of EVs over tissue biopsies for biomarker detection.
- To emphasize the potential of EV-based proteomics and phosphoproteomics in clinical settings.
Main Methods:
- Large-scale proteome and phosphoproteome analyses using liquid chromatography and mass spectrometry.
- Utilizing extracellular vesicles (EVs) for biomarker sample collection.
- Development of clinically-friendly EV preparation techniques.
Main Results:
- EVs provide stable preservation of labile phosphoproteins, crucial for biomarker detection.
- EVs offer a non-invasive alternative to tissue biopsies for accessing protein biomarkers.
- Advancements in EV analysis techniques facilitate diagnostic and therapeutic applications.
Conclusions:
- Extracellular vesicles (EVs) represent a significant advancement for non-invasive liquid biopsy.
- EV proteomics and phosphoproteomics hold great potential for routine clinical biomarker detection.
- Streamlined EV analysis will likely become a standard in clinical practice for disease management.
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