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Updated: Aug 14, 2025

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Pump-less, recirculating organ-on-a-chip (rOoC) platform.

Mathias Busek1,2, Aleksandra Aizenshtadt1, Timo Koch3

  • 1Hybrid Technology Hub - Centre of Excellence, Institute of Basic Medical Science, University of Oslo, P.O. Box 1110, 0317 Oslo, Norway.

Lab on a Chip
|January 19, 2023
PubMed
Summary

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This summary is machine-generated.

We created a novel, pump-less organ-on-a-chip platform using gravity-driven flow for advanced tissue engineering. This system supports complex co-cultures, including endothelial cells and liver organoids, with circulating immune cells.

Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Tissue Engineering

Background:

  • Organ-on-a-chip (OoC) platforms are crucial for modeling human physiology.
  • Existing OoC systems often rely on external pumps, complicating integration and scalability.
  • Controlled microfluidic flow is essential for mimicking in vivo conditions.

Purpose of the Study:

  • To develop a novel, pump-less recirculating organ-on-a-chip (rOoC) platform.
  • To enable controlled, unidirectional gravity-driven flow for enhanced tissue culture.
  • To demonstrate the platform's capability for multi-tissue integration and cell circulation.

Main Methods:

  • Utilized layer-to-layer fabrication with thermoplastic materials for the rOoC platform.
  • Implemented a 3D-tilting system and optimized microfluidic layout for gravity-driven flow.

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  • Employed computational modeling and micro-Particle-Image-Velocimetry (μPIV) to characterize flow dynamics.
  • Assessed endothelial cell (HUVEC) function, liver organoid viability, and immune cell circulation.

    • Main Results:

    • Successfully established a pump-less, gravity-driven flow system in the rOoC.
    • Demonstrated HUVEC alignment, barrier formation, and sprouting in response to flow.
    • Confirmed the viability of human stem-cell derived liver organoids within the platform.
    • Showcased circulation of viable immune cells without trapping or activation.

    Conclusions:

    • The developed rOoC platform offers a novel approach for pump-less, controlled microfluidic perfusion.
    • It supports the functional culture and integration of multiple organoids and cell types.
    • This technology facilitates the creation of complex, vascularized tissue models with circulating components for advanced research.