Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

430
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI spectrometry is widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.
The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix material. The...
430

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Label-free fluorescence displacement sensors based on split aptamers and Thioflavin T for the rapid and sensitive detection of kanamycin in milk.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment·2025
Same author

Recent Advances in Nanomaterial-Based Chemiluminescence Probes for Biosensing and Imaging of Reactive Oxygen Species.

Nanomaterials (Basel, Switzerland)·2023
Same author

Label-free microRNA detection using a locked-to-unlocked transforming system assembled by microfluidics.

Lab on a chip·2022
Same author

Fluorinated covalent organic frameworks as a stationary phase for separation of fluoroquinolones by capillary electrochromatography.

Mikrochimica acta·2022
Same author

Multilayer Ratiometric Fluorescent Nanomachines for Imaging mRNA in Live Cells.

Small methods·2021
Same author

Adsorption behavior and mechanism of sulfonamides on controllably synthesized covalent organic frameworks.

Environmental science and pollution research international·2021

Related Experiment Video

Updated: Aug 13, 2025

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
09:08

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

Published on: December 22, 2020

6.3K

Systematic study of tissue section thickness for MALDI MS profiling and imaging.

Xiaofei Wang1, Lu Zhang1, Yuhong Xiang1

  • 1Department of Chemistry, Capital Normal University, Beijing 100048, China. yensh@cnu.edu.cn.

The Analyst
|January 20, 2023
PubMed
Summary

Tissue slice thickness significantly impacts Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) quality. Optimal brain tissue thickness is 2-6 μm, with atmospheric pressure MALDI showing greater tolerance than vacuum MALDI.

More Related Videos

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

14.3K
MALDI Sample Preparation: the Ultra Thin Layer Method
05:28

MALDI Sample Preparation: the Ultra Thin Layer Method

Published on: April 29, 2007

19.5K

Related Experiment Videos

Last Updated: Aug 13, 2025

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
09:08

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

Published on: December 22, 2020

6.3K
Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

14.3K
MALDI Sample Preparation: the Ultra Thin Layer Method
05:28

MALDI Sample Preparation: the Ultra Thin Layer Method

Published on: April 29, 2007

19.5K

Area of Science:

  • Biomedical Imaging
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) is crucial for molecular spatial distribution analysis.
  • Tissue section preparation is a critical determinant of MALDI MSI data quality.
  • The impact of tissue slice thickness on MALDI MSI outcomes remains under-explored.

Purpose of the Study:

  • To systematically investigate the effect of tissue slice thickness on MALDI MSI detection and data quality.
  • To determine optimal tissue slice thicknesses for various MALDI MSI applications.
  • To compare the influence of slice thickness on atmospheric pressure (AP) and vacuum MALDI assays.

Main Methods:

  • MALDI MSI was performed on tissue sections of varying thicknesses (400 nm to 150 μm).
  • Ion signals and imaging quality were analyzed across different slice thicknesses.
  • Peptide detection after enzymatic digestion of tissue sections was evaluated.
  • Optimized slice thicknesses were determined for six different mouse tissues.

Main Results:

  • Thicker tissue slices generally resulted in poorer MALDI MSI quality, attributed to a charging effect.
  • Optimal brain tissue slice thickness for MALDI MSI was found to be 2-6 μm.
  • Atmospheric pressure (AP) MALDI exhibited greater tolerance to slice thickness variations compared to vacuum MALDI.
  • A tissue thickness of approximately 10 μm was optimal for enzymatic digestion in MALDI MSI.

Conclusions:

  • Tissue slice thickness is a critical parameter that must be optimized for high-quality MALDI MSI.
  • AP MALDI offers advantages in tolerance to slice thickness variations over vacuum MALDI.
  • These findings provide essential references for optimizing sample preparation in MALDI MSI studies.