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Protocol for UVC uridine actinometry.

Dana Pousty¹, Hadas Mamane¹, Vered Cohen-Yaniv¹

¹School of Mechanical Engineering, Faculty of Engineering, Tel-Aviv University, Tel-Aviv, Israel.

|January 23, 2023

Summary

This summary is machine-generated.

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Uridine actinometry offers a simple, eco-friendly method for measuring germicidal UV light. This technique is ideal for validating the photon flux of UVC disinfection systems, including both mercury lamps and UV-LEDs.

Area of Science:

Photochemistry
Microbiology
Environmental Science

Background:

Uridine, containing the uracil chromophore, shares absorption spectra with DNA between 240-290 nm.
This spectral overlap aligns with the inactivation sensitivity of most microorganisms to UV light.
Uridine's absorption maximum at 262 nm makes it suitable for measuring germicidal photon flux.

Purpose of the Study:

To present uridine actinometry as an ideal method for determining germicidal photon flux.
To validate the photon flux of Ultraviolet C (UVC) disinfection systems.
To offer a simple, environmentally friendly, and user-friendly actinometry protocol.

Main Methods:

Uridine actinometry utilizes the absorbance spectrum of uridine to quantify UV light.

Keywords:

UV-LED UVC actinometry UVC uridine actinometry Uridine actinometry

Related Experiment Videos

The protocol is designed for accuracy and ease of operation.

It is adaptable for various UVC sources, including low-pressure mercury lamps and UV-LEDs.

Main Results:

Uridine actinometry is highly effective for validating the photon flux of UVC systems.
The method is suitable for both conventional low-pressure mercury lamps and emerging UV-LED disinfection technologies.
Initial uridine concentration and photoproduct absorbance influence kinetic order and quantum yield, allowing for protocol customization.

Conclusions:

Uridine actinometry provides an accurate, simple, and environmentally sound approach for UVC system validation.
Its applicability to both UV-LP and UV-LED sources makes it versatile for water disinfection.
The protocol's customizable parameters ensure precise photon flux determination.