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RNA Structure Determination by High-Throughput Structural Analysis.
1Laboratory of Virology, Institute of Microbial Chemistry (BIKAKEN), Tokyo, Japan. takizawan@bikaken.or.jp.
Methods in Molecular Biology (Clifton, N.J.)
|January 27, 2023
Summary
Understanding RNA secondary structure is key to RNA function. High-throughput RNA structural analyses now profile RNA structures across the transcriptome in living cells, with computational pipelines like PROBer, BUMHMM, and reactIDR aiding data processing.
Area of Science:
- Molecular Biology
- Genomics
- Bioinformatics
Background:
- RNA function is intrinsically tied to its secondary structure.
- Experimental methods like chemical modification and RNase digestion reveal RNA structures.
- Advances in sequencing and chemical analysis enable transcriptomic-scale RNA structural profiling in vivo.
Purpose of the Study:
- To provide an overview of high-throughput RNA structural (HTS) analyses.
- To describe the computational processing steps for HTS analysis pipelines.
Main Methods:
- Overview of high-throughput RNA structural (HTS) analyses.
- Description of computational processing for PROBer, BUMHMM, and reactIDR pipelines.
Main Results:
- HTS analyses offer transcriptomic-scale RNA structural profiling in living cells.
- Computational pipelines facilitate the processing of HTS data.
Conclusions:
- Elucidating RNA secondary structures is crucial for understanding RNA function.
- HTS analyses and associated computational tools advance the study of RNA structure-function relationships.
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