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A cell type specific factor recognizes the rat thyroglobulin promoter.

A M Musti1, V M Ursini, E V Avvedimento

  • 1Centro di Endocrinologia ed Oncologia sperimentale del CNR, II Facoltà di Medicina, Napoli, Italy.

Nucleic Acids Research
|October 26, 1987
PubMed
Summary
This summary is machine-generated.

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Researchers identified a specific DNA region in the rat thyroglobulin (Tg) gene promoter essential for thyroid cell-specific gene expression. This region binds a thyroid-specific nuclear factor, crucial for regulating Tg gene activity.

Area of Science:

  • Molecular Biology
  • Genetics
  • Endocrinology

Background:

  • Thyroglobulin (Tg) is a key protein in thyroid hormone synthesis.
  • Understanding the regulation of Tg gene expression is crucial for thyroid biology.

Purpose of the Study:

  • To identify the DNA elements responsible for cell-type specific expression of the rat thyroglobulin (Tg) gene.
  • To investigate the role of nuclear factors in mediating this cell-specific regulation.

Main Methods:

  • Gene fusion of the rat Tg promoter with the chloramphenicol acetyltransferase (cat) reporter gene.
  • Transfection into rat thyroid and liver cell lines.
  • Deletion analysis of the Tg promoter sequence.
  • Electrophoretic mobility shift assays (EMSA) to detect nuclear factor binding.

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Main Results:

  • The fusion gene was expressed specifically in a thyroid cell line that also expresses the endogenous Tg gene.
  • Deletion analysis identified a 170-nucleotide region upstream of the transcription start site as critical for cell-specific expression.
  • A thyroid cell-specific nuclear factor binding site was localized within this critical region.

Conclusions:

  • A minimal promoter region of the rat Tg gene confers thyroid cell-specific expression.
  • A thyroid-specific nuclear factor binding to this region likely mediates cell-type specific gene regulation.