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Construction of Human Three-Dimensional Lung Model Using Layer-by-Layer Method.

Yukako Akamatsu1,2, Takami Akagi3, Tomoko Sumitomo1

  • 1Department of Oral and Molecular Microbiology, Osaka University Graduate School of Dentistry, Osaka, Japan.

Tissue Engineering. Part C, Methods
|February 1, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel 3D human lung model using a layer-by-layer cell coating technique. This advanced model mimics lung tissue, offering improved insights into pathogen interactions and respiratory diseases like pneumonia.

Keywords:
3D lung modelcell coating techniquelayer-by-layer

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Area of Science:

  • Biomedical Engineering
  • Respiratory Medicine
  • Cell Biology

Background:

  • Lung epithelial barrier integrity is crucial for defense against respiratory pathogens.
  • Existing 2D cell cultures and animal models have limitations in evaluating human-specific host-pathogen interactions.
  • There is a need for advanced 3D in vitro lung models to study diseases like pneumonia and viral infections.

Purpose of the Study:

  • To develop a novel, functional three-dimensional (3D) human lung model for studying pathogen-host interactions.
  • To utilize a layer-by-layer (LbL) cell coating technique for constructing a physiologically relevant lung tissue model.
  • To assess the epithelial polarity and barrier function of the developed 3D lung model.

Main Methods:

  • Fabrication of a 3D lung model using a layer-by-layer (LbL) cell coating technique.
  • Construction involved coating human primary pulmonary fibroblast cells with fibronectin (FN) and gelatin (G).
  • A monolayer of Calu-3 human lower airway epithelial cells was placed on the 3D fibroblast scaffold.
  • Characterization included immunohistochemical staining for E-cadherin, ZO-1, and mucin, and measurement of transepithelial electrical resistance (TEER).

Main Results:

  • The constructed 3D lung model maintained uniform thickness for up to 7 days of incubation.
  • Immunohistochemical analysis confirmed the expression of key epithelial markers (E-cadherin, ZO-1, mucin).
  • TEER measurements indicated the presence of epithelial barrier function, similar to native lung tissue.

Conclusions:

  • The developed 3D human lung model effectively replicates key features of native lung tissue, including epithelial polarity and barrier function.
  • This novel LbL-based model provides a valuable platform for investigating infection mechanisms and pathological processes in respiratory diseases.
  • The model is suitable for studying interactions between various pathogens and human lung biology.