Challenges and perspectives of multi-virus biosensing techniques: A review

Area of Science:

• Biomedical Engineering
• Nanotechnology
• Infectious Disease Diagnostics

Background:

• Globalization increases the risk of simultaneous viral infections, necessitating advanced diagnostic tools.
• Current diagnostic methods often lack the capacity for simultaneous detection of multiple pathogens.
• Multiplexed biosensing offers a solution for rapid, high-throughput, and parallel analysis of viral infections.

Purpose of the Study:

• To review and analyze optical, electrochemical, and mechanical platforms for multi-virus biosensing.
• To evaluate the working mechanisms, advantages, limitations, and real-life implementation challenges of these platforms.
• To discuss future perspectives in multiplexed biosensing for infectious disease control.

Main Methods:

• Review of existing literature on optical, electrochemical, and mechanical biosensing platforms.
• Analysis of detection principles, transducer configurations, bio-interface designs, and signal detection.
• Evaluation of platform performance, scalability, and applicability in clinical settings.

Main Results:

• Optical, electrochemical, and mechanical platforms demonstrate potential for multi-virus detection with varying strengths and weaknesses.
• Key factors influencing performance include sensitivity, response time, throughput, and automation capabilities.
• Challenges remain in real-world implementation, including cost, standardization, and integration into existing healthcare systems.

Conclusions:

• Multiplexed biosensors are essential for efficient detection of multiple viruses, aiding in immediate pandemic response.
• Further development is needed to overcome implementation challenges and realize the full potential of these technologies.
• Advancements in multiplexed biosensing will be critical for managing emerging infectious diseases like SARS-CoV-2 and monkeypox.