Development of small-sized fluorescence detector for pipette tip-based biosensor for on-site diagnosis
Masakazu Kagawa1, Kazuhiro Morioka2, Moeko Osashima1
1Department of Applied Chemistry for Environment, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, 1-1 Minami-osawa, Hachioji, Tokyo, 192-0397, Japan.
Talanta
|February 4, 2023
Summary
A novel pipette tip (PT)-reader was developed for biosensors, enabling sensitive fluorescence detection. This compact device offers comparable performance to larger readers and facilitates on-site medical diagnostics.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Sensor Technology
Background:
- Existing fluorescence detection methods often require bulky and expensive equipment.
- There is a need for portable and cost-effective solutions for on-site diagnostics.
Purpose of the Study:
- To develop a small-sized fluorescence detector, the pipette tip (PT)-reader, for pipette tip-based biosensors.
- To evaluate the performance of the PT-reader for sensitive and accurate fluorescence measurements.
- To demonstrate the utility of the PT-reader in quantifying biomarkers for on-site medical diagnosis.
Main Methods:
- Development of a compact PT-reader integrated with a specialized pipette holder for precise tip positioning and stray light prevention.
- Characterization of the PT-reader's performance using resorufin fluorescence measurements.
- Application of the PT-reader for quantifying immunoglobulin A (IgA) using a PT-based enzyme-linked immunosorbent assay (PT-ELISA).
Main Results:
- The PT-reader achieved a limit of detection (LOD) of 0.46 μM for resorufin with a relative standard deviation (RSD) of 0.47-4.1%.
- Performance was comparable to desktop-type fluorescence microplate readers.
- The PT-reader demonstrated an LOD of 1.0 ng/mL for IgA, with results from PT-ELISA in human saliva correlating well with conventional ELISA.
Conclusions:
- The developed PT-reader is a sensitive, low-cost, and user-friendly fluorescence detection tool.
- The PT-reader shows significant potential for advancing on-site medical diagnostics.
- The device development strategy contributes to the progress of portable diagnostic technologies.
