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Updated: Aug 11, 2025

In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS
Published on: February 15, 2016
Surface or Internal Hydration - Does It Really Matter?
Christian van der Linde1, Milan Ončák1, Ethan M Cunningham1
1Institut für Ionenphysik und Angewandte Physik, Universität Innsbruck, Technikerstraße 25, 6020Innsbruck, Austria.
Abstract:
The precise location of an ion or electron, whether it is internally solvated or residing on the surface of a water cluster, remains an intriguing question. Subtle differences in the hydrogen bonding network may lead to a preference for one or the other. Here we discuss spectroscopic probes of the structure of gas-phase hydrated ions in combination with quantum chemistry, as well as H/D exchange as a means of structure elucidation. With the help of nanocalorimetry, we look for thermochemical signatures of surface vs internal solvation. Examples of strongly size-dependent reactivity are reviewed which illustrate the influence of surface vs internal solvation on unimolecular rearrangements of the cluster, as well as on the rate and product distribution of ion-molecule reactions.
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