Melaminivora suipulveris sp. nov., isolated from pigpen dust

A bacterial strain designated SC2-9^T was isolated from the dust collector of a pigpen located in Wanju-gun, Jeollabuk-do, Republic of Korea. Cells were strictly aerobic, Gram-stain-negative, flagellated and rod-shaped. The strain was catalase- and oxidase-positive, and grew optimally 28-30 °C, pH 8.0 and 0 % NaCl (w/v). Phylogenetic analysis based on 16S rRNA gene sequences showed 99.1 and 98.3 % similarities to Melaminivora jejuensis KBB12^T and Melaminivora alkalimesophila CY1^T, and revealing less than 97 % similarity to other validly named species. The genomic DNA G+C content of strain SC2-9^T was 68.2 %. The orthologous average nucleotide identity and dDDH values of strain SC2-9^T with the closest species Melaminivora jejuensis KCTC 32230^T were 85.6 and 29.3 %, respectively. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified aminolipids and one unidentified lipid. The major fatty acids (>10 %) were summed feature 3 (C_16 : 1  ω6c and/or C_16 : 1  ω7c), C_16 : 0 and summed feature 8 (C_18 : 1  ω6c and/ or C_18 : 1  ω7c). The predominant isoprenoid quinone was ubiquinone-8. Based on phenotypic, chemotaxonomic and phylogenetic data, strain SC2-9^T should be assigned as a novel species of the genus Melaminivora, for which the name Melaminivora suipulveris sp. nov. is proposed. The type strain is SC2-9^T (=KACC 19310^T=NBRC 113103^T).